8DB2
Q108K:K40L:T51C:T53A:R58L:Q38F mutant of hCRBPII bound to synthetic fluorophore CM1V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-27 |
Detector | RIGAKU HyPix-3000 |
Wavelength(s) | 1.54184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 29.075, 66.292, 63.618 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.590 - 1.500 |
R-factor | 0.2292 |
Rwork | 0.225 |
R-free | 0.26810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qyn |
RMSD bond length | 0.006 |
RMSD bond angle | 0.880 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.590 | 1.560 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.460 | |
Number of reflections | 18253 | 1828 |
<I/σ(I)> | 24.78 | 0.76 |
Completeness [%] | 95.8 | 83.9 |
Redundancy | 2.3 | 1.5 |
CC(1/2) | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 297 | PEG 4000, Ammonium Acetate, Sodium Acetate, pH 4.0 - 4.8 |