8DAM

nbF3:nbE8:CaV beta subunit 1b complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1159
Spacegroup name	P 21 21 21
Unit cell lengths	80.358, 81.072, 93.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.690 - 2.000
R-factor	0.1948
Rwork	0.193
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v2p
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.690	2.072
High resolution limit [Å]	2.000	2.000
Number of reflections	41850	4114
<I/σ(I)>	17.53
Completeness [%]	98.9
Redundancy	12.7
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	15-22% PEG3350, 0.15-0.25M Ammonium Sulphate