8DAJ
Structure and Biochemistry of a Promiscuous Thermophilic Polyhydroxybutyrate Depolymerase from Lihuaxuella thermophilia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-12-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 45.510, 45.510, 211.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.930 - 1.200 |
| R-factor | 0.1637 |
| Rwork | 0.163 |
| R-free | 0.17720 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.961 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.930 | 1.243 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.134 | 0.473 |
| Rmeas | 0.138 | 0.517 |
| Rpim | 0.032 | 0.199 |
| Number of reflections | 6485 | |
| <I/σ(I)> | 11.84 | 1.51 |
| Completeness [%] | 97.7 | 81.8 |
| Redundancy | 16.7 | 6 |
| CC(1/2) | 0.997 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.1M citrate pH 5.6, 18% propanol, and 20% PEG 4000 |
