8D89

Crystal structure of a novel GH5 enzyme retrieved from capybara gut metagenome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRUS BEAMLINE MANACA
Synchrotron site	LNLS SIRUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458640
Spacegroup name	P 21 21 2
Unit cell lengths	120.600, 58.860, 60.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.765 - 1.600
R-factor	0.172
Rwork	0.171
R-free	0.19410
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.765	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	54691	5045
<I/σ(I)>	10.12
Completeness [%]	94.7
Redundancy	12.61
CC(1/2)	0.990	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	19% (w/v) PEG 600, 0.1 M sodium acetate pH 5.0, and 0.2 M of magnesium chloride