8D7O

Human Casein kinase 1 delta in complex with phosphorylated human PERIOD2 FASP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	C 1 2 1
Unit cell lengths	55.330, 135.400, 90.351
Unit cell angles	90.00, 94.41, 90.00

Refinement procedure

Resolution	54.120 - 1.650
R-factor	0.1743
Rwork	0.172
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pxo
RMSD bond length	0.015
RMSD bond angle	1.255
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.700	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.064	0.542
Number of reflections	78946	4059
<I/σ(I)>	13.6	2.9
Completeness [%]	99.4
Redundancy	5.9
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.13M Succinic Acid (5.5) 27%PEG3350