8D7N

Human Casein kinase 1 delta in complex with phosphorylated human PERIOD2 FASP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	C 1 2 1
Unit cell lengths	55.900, 136.030, 90.540
Unit cell angles	90.00, 94.60, 90.00

Refinement procedure

Resolution	51.562 - 1.660
R-factor	0.1784
Rwork	0.177
R-free	0.20610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pxo
RMSD bond length	0.011
RMSD bond angle	1.261
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.010	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.093	0.556
Number of reflections	79274	3942
<I/σ(I)>	9.9	2.7
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M Succinic Acid (5.5) 15% PEG 3350