8D6N

Q108K:K40L:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1272
Spacegroup name	C 1 2 1
Unit cell lengths	29.612, 67.106, 63.903
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	33.550 - 1.420
R-factor	0.2145
Rwork	0.213
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qyp
RMSD bond length	0.007
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.550	1.470
High resolution limit [Å]	1.420	1.420
Rmeas	0.036	0.198
Rpim	0.014	0.079
Number of reflections	22948	2284
<I/σ(I)>	22949
Completeness [%]	97.3	96.21
Redundancy	6.4
CC(1/2)		0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	297	PEG 4000, Ammonium Acetate, Sodium Acetate, pH 4.0 - 4.8