8D6L

Q108K:K40L:T51C:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1272
Spacegroup name	C 1 2 1
Unit cell lengths	29.613, 67.250, 63.687
Unit cell angles	90.00, 92.28, 90.00

Refinement procedure

Resolution	33.620 - 1.690
R-factor	0.24
Rwork	0.235
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qyp
RMSD bond length	0.024
RMSD bond angle	1.627
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.630	1.749
High resolution limit [Å]	1.690	1.690
Rmeas	0.041	0.488
Rpim	0.013	0.205
Number of reflections	13067	1152
<I/σ(I)>	13067
Completeness [%]	93.0	81.7
Redundancy	9.8
CC(1/2)		0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	297	PEG 4000, Ammonium Acetate, Sodium Acetate, pH 4.0 - 4.8