8D6G
Nanorana parkeri saxiphilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033167 |
Spacegroup name | H 3 |
Unit cell lengths | 229.046, 229.046, 67.428 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.290 - 2.200 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.23540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o0d |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.290 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.052 | |
Number of reflections | 66067 | 6127 |
<I/σ(I)> | 12.66 | 0.89 |
Completeness [%] | 98.6 | |
Redundancy | 3.3 | |
CC(1/2) | 0.999 | 0.465 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277.15 | 20-25% v/v PEG400, 4-5% w/v PGA-LM, 100-200 mM sodium acetate, pH 5.0 |