8D60

Crystal structure of hen egg white lysozyme at 150 Kelvin

Experimental procedure

Experimental method	LAUE
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	150
Detector technology	CMOS
Collection date	2020-10-13
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	78.719, 78.719, 37.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.286 - 1.500
Rwork	0.170
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a8z
RMSD bond length	0.013
RMSD bond angle	1.880
Data reduction software	SAINT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.290	22.290
High resolution limit [Å]	1.500	1.500
Number of reflections	19294	18306
<I/σ(I)>	9.58
Completeness [%]	99.7
Redundancy	1.9
CC(1/2)	0.736	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	1.2 M NaCl and 100 mM AcONa pH 4.6