8D5W

Crystal structure of hen egg white lysozyme at 125 Kelvin

Experimental procedure

Experimental method	LAUE
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	125
Detector technology	CMOS
Collection date	2020-10-07
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	78.782, 78.782, 37.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.300 - 1.499
Rwork	0.185
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a8z
RMSD bond length	0.012
RMSD bond angle	1.811
Data reduction software	SAINT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.300	22.300
High resolution limit [Å]	1.499	1.499
Number of reflections	19280	19280
<I/σ(I)>	7.17
Completeness [%]	99.6
Redundancy	1.89
CC(1/2)	0.802	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	1.2 M NaCl and 100 mM AcONa pH 4.6