8D5V
WhiB6 bound to the SigmaAr4-RNAP Beta flap tip chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.983, 66.540, 158.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.270 - 1.800 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.23060 |
| Structure solution method | SAD |
| Starting model (for MR) | 6ono |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 1.590 |
| Rmeas | 0.070 | 1.652 |
| Rpim | 0.020 | 0.442 |
| Number of reflections | 30736 | 3011 |
| <I/σ(I)> | 17.96 | 1.51 |
| Completeness [%] | 97.4 | 97.53 |
| Redundancy | 12.7 | 13.6 |
| CC(1/2) | 0.999 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M Na Citrate pH 5.5, 20% PEG3000 |
