8D50

Crystal Structure of Mosaic HIV-1 Envelope (MosM3.1) in Complex with antibodies PGT124 and 35O22 at 4.3 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03321
Spacegroup name	P 63
Unit cell lengths	132.189, 132.189, 315.415
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.270 - 4.320
R-factor	0.3252
Rwork	0.323
R-free	0.36910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.270	50.000	4.370
High resolution limit [Å]	4.300	11.630	4.300
Rmerge	0.228	0.044	0.899
Rmeas	0.252	0.048	1.002
Rpim	0.104	0.018	0.428
Number of reflections	19809	1071	874
<I/σ(I)>	4.1
Completeness [%]	95.3	99.4	83.7
Redundancy	5.4	6.8	4.2
CC(1/2)		0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M imidazole (pH=7.33), 0.2M lithium sulfate, 8.545%(w/v) PEG3000