8D4R

Crystal Structure of Mosaic HIV-1 Envelope (MosM3.2) in Complex with antibodies PGT124 and 35O22 at 3.8 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03316
Spacegroup name	P 63
Unit cell lengths	125.474, 125.474, 315.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.190 - 3.810
R-factor	0.2788
Rwork	0.277
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.870
High resolution limit [Å]	3.800	10.290	3.800
Rmerge	0.163	0.036	1.206
Rmeas	0.188	0.042	1.425
Rpim	0.093	0.020	0.747
Total number of observations	105995
Number of reflections	27342	1365	1327
<I/σ(I)>	5.1
Completeness [%]	99.3	96.6	98.7
Redundancy	3.9	3.9	3.4
CC(1/2)		0.998	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris (pH=7.4), 23% v/v PEG 400