8D4I

Structure of Y430F D-ornithine/D-lysine decarboxylase complex with putrescine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	140.880, 50.440, 139.180
Unit cell angles	90.00, 116.93, 90.00

Refinement procedure

Resolution	41.360 - 1.320
R-factor	0.1463
Rwork	0.146
R-free	0.17090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6N2H.pdb
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.360	1.367
High resolution limit [Å]	1.320	1.320
Number of reflections	197103	15354
<I/σ(I)>	11.63
Completeness [%]	96.3
Redundancy	9.6
CC(1/2)	0.999	0.071

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES, pH 7.5, 0.2 M NaOAc, 20% PEG 4000