8D40

Crystal structure of human CELSR1 EC1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9798
Spacegroup name	C 1 2 1
Unit cell lengths	305.653, 90.356, 94.949
Unit cell angles	90.00, 96.17, 90.00

Refinement procedure

Resolution	47.244 - 3.554
Rwork	0.216
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v5x 5w4t 3q2v 3q2w
RMSD bond length	0.010
RMSD bond angle	1.932
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.244	3.610
High resolution limit [Å]	3.550	3.550
Number of reflections	31176	1550
<I/σ(I)>	10.094	2.5
Completeness [%]	98.7	98.2
Redundancy	5.7	5.3
CC(1/2)	0.993	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	3 M Sodium Chloride, 0.1 M Tris pH 8.5