8D2J
A novel insecticidal protein from ferns IPD113_Cow.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.641, 79.393, 108.548 |
| Unit cell angles | 97.92, 104.95, 97.41 |
Refinement procedure
| Resolution | 47.820 - 2.100 |
| R-factor | 0.22148 |
| Rwork | 0.220 |
| R-free | 0.25557 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 138352 | 5669 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.997 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | conditions F3, F8, H4, H5, H6 and H8 of the Index HTTM screen |
