8D19
Human LanCL1 bound to GSH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-01-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.505, 121.134, 142.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.520 |
| R-factor | 0.17314 |
| Rwork | 0.172 |
| R-free | 0.19231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e6u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.020 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.401 | 1.517 |
| High resolution limit [Å] | 1.512 | 1.512 |
| Rmerge | 0.079 | 1.088 |
| Rmeas | 0.082 | 1.142 |
| Rpim | 0.022 | 0.341 |
| Number of reflections | 150903 | 1476 |
| <I/σ(I)> | 20.6 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.4 | 10.7 |
| CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 282.15 | 0.7-0.75 M succinic acid pH 7.0, 0.1 M bis-tris propane pH 7.0 |
