8D19

Human LanCL1 bound to GSH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-01-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	55.505, 121.134, 142.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.520
R-factor	0.17314
Rwork	0.172
R-free	0.19231
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6u
RMSD bond length	0.003
RMSD bond angle	1.020
Data reduction software	AutoProcess
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.401	1.517
High resolution limit [Å]	1.512	1.512
Rmerge	0.079	1.088
Rmeas	0.082	1.142
Rpim	0.022	0.341
Number of reflections	150903	1476
<I/σ(I)>	20.6	2.1
Completeness [%]	100.0	100
Redundancy	14.4	10.7
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		282.15	0.7-0.75 M succinic acid pH 7.0, 0.1 M bis-tris propane pH 7.0