8D09
Hallucinated C4 protein assembly HALC4_136
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.829, 77.894, 52.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.340 - 1.900 |
| R-factor | 0.2329 |
| Rwork | 0.232 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Design model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.340 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 8869 | 875 |
| <I/σ(I)> | 9.45 | 3.95 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 13.1 | |
| CC(1/2) | 0.981 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M SPG buffer pH 5 25% w/v PEG 1500 |
