8D08

Hallucinated C4 protein assembly HALC4_135

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97926
Spacegroup name	P 41 21 2
Unit cell lengths	35.867, 35.867, 437.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.750 - 3.300
R-factor	0.2526
Rwork	0.250
R-free	0.29790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Design model
RMSD bond length	0.011
RMSD bond angle	1.370
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.750	3.418
High resolution limit [Å]	3.300	3.300
Number of reflections	8009	435
<I/σ(I)>	9.29	1.37
Completeness [%]	98.7	95.19
Redundancy	6.8	6.4
CC(1/2)	0.996	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Lithium sulfate monohydrate 0.1 M BIS-TRIS 25% w/v Polyethylene glycol 3,350