8D07
Hallucinated C3 protein assembly HALC3_109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.825, 35.638, 94.417 |
| Unit cell angles | 90.00, 129.74, 90.00 |
Refinement procedure
| Resolution | 72.610 - 2.090 |
| R-factor | 0.2091 |
| Rwork | 0.206 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Design model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.610 | 2.165 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 20957 | 2047 |
| <I/σ(I)> | 11.77 | 2.06 |
| Completeness [%] | 98.5 | 99.27 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.995 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% w/v PEG 20000 20% v/v PEG MME 550 0.02M amino acid mix 0.1M MOPS/HEPES-Na (Buffer system 2) |
