8D06

Hallucinated C3 protein assembly HALC3_104

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97926
Spacegroup name	P 41
Unit cell lengths	107.496, 107.496, 111.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.450 - 3.400
R-factor	0.2852
Rwork	0.284
R-free	0.30860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Design model
RMSD bond length	0.003
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.450	3.522
High resolution limit [Å]	3.400	3.400
Number of reflections	17541	1749
<I/σ(I)>	14.56	2.61
Completeness [%]	99.4	99.43
Redundancy	4.8
CC(1/2)	0.999	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.05 M Cesium chloride 0.1 M MES 30% Jeffamine M-600