8D05

Hallucinated C2 protein assembly HALC2_065

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 42
Unit cell lengths	50.187, 50.187, 22.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.190 - 2.510
R-factor	0.2385
Rwork	0.237
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Design model
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.190	2.601
High resolution limit [Å]	2.510	2.510
Number of reflections	2002	193
<I/σ(I)>	2.85
Completeness [%]	99.9	100
Redundancy	13.2	14
CC(1/2)	0.987	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	293	0.2 M Ammonium nitrate 20% PEG 3350