8D04

Hallucinated C2 protein assembly HALC2_062

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97926
Spacegroup name	P 65
Unit cell lengths	67.910, 67.910, 228.407
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.810 - 2.110
R-factor	0.2389
Rwork	0.237
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Design model
RMSD bond length	0.003
RMSD bond angle	0.514
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.810	2.186
High resolution limit [Å]	2.110	2.110
Number of reflections	34088	3405
<I/σ(I)>	16.65
Completeness [%]	99.8	99.47
Redundancy	7.1
CC(1/2)	0.996	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M Bis-Tris 2.0 M Ammonium sulfate