8D03

Hallucinated C2 protein assembly HALC2_068

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	70.635, 70.635, 31.382
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.900 - 1.750
R-factor	0.191
Rwork	0.190
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Design model
RMSD bond length	0.006
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.900	1.813
High resolution limit [Å]	1.750	1.751
Number of reflections	9287	819
<I/σ(I)>	8.85	1.33
Completeness [%]	98.9	89.99
Redundancy	9.8
CC(1/2)	0.950	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.04 M Potassium phosphate 16% PEG 8000