8CZL

Human LanCL1 bound to methyl glutathione (MeGSH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-12
Detector	RAYONIX MX-300
Wavelength(s)	0.978720
Spacegroup name	P 21 21 21
Unit cell lengths	55.485, 120.909, 143.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.580
R-factor	0.18226
Rwork	0.181
R-free	0.20388
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6u
RMSD bond length	0.003
RMSD bond angle	1.069
Data reduction software	AutoProcess
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.357	1.605
High resolution limit [Å]	1.577	1.577
Rmerge	0.084	0.959
Rmeas	0.088	1.023
Rpim	0.029	0.348
Number of reflections	130838	6255
<I/σ(I)>	17.1	2.1
Completeness [%]	98.3
Redundancy	9.4
CC(1/2)	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		282.15	0.70-0.75 M succinic acid pH 7.0, 0.1 M bis-tris-propane pH 7.0.