8CZK

Human LanCL1 bound to GSH and Dhb-Erk peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	55.688, 120.562, 144.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.910
R-factor	0.18542
Rwork	0.184
R-free	0.21338
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6u
RMSD bond length	0.004
RMSD bond angle	1.079
Data reduction software	AutoProcess
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.500	1.920
High resolution limit [Å]	1.910	1.914
Rmerge	0.119	1.284
Rmeas	0.126	1.360
Rpim	0.041	0.438
Number of reflections	75709	809
<I/σ(I)>	13.5	2.1
Completeness [%]	100.0	100
Redundancy	10	10.1
CC(1/2)	0.998	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	282	0.70-0.75 M succinic acid pH 7.0, 0.1 M bis-tris-propane pH 7.0