8CZJ

A bacteria Zrt/Irt-like protein in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	193
Detector technology	PIXEL
Collection date	2019-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	62.985, 117.661, 64.290
Unit cell angles	90.00, 104.16, 90.00

Refinement procedure

Resolution	37.109 - 2.751
R-factor	0.2352
Rwork	0.233
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsb
RMSD bond length	0.010
RMSD bond angle	1.310
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.109	3.110
High resolution limit [Å]	2.751	3.001
Rmeas	0.194
Rpim	0.088	0.289
Number of reflections	22227	17721
<I/σ(I)>	6.7
Completeness [%]	97.3
Redundancy	3.6
CC(1/2)	0.772	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	294	27%PEG200,0.02M NaAc 4.0,0.2M(NH4)2SO4,0.02M NaCl