8CZF

Human BAK in complex with the dF2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	94.130, 41.080, 56.390
Unit cell angles	90.00, 122.00, 90.00

Refinement procedure

Resolution	47.820 - 1.300
R-factor	0.1622
Rwork	0.161
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vx0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.820	47.820	1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.033	0.018	0.588
Rmeas	0.036	0.019	0.653
Total number of observations	289866
Number of reflections	43728	542	2462
<I/σ(I)>	25.36	88	2.81
Completeness [%]	96.6	98	73.5
Redundancy	6.629	6.614	5.277
CC(1/2)	1.000	1.000	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.2 M sodium malonate