8CZ5

N11 P domain 2D9 Fab P complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	291.15
Detector technology	PIXEL
Collection date	2017-10-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	P 65 2 2
Unit cell lengths	92.290, 92.290, 493.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.807 - 2.645
R-factor	0.1953
Rwork	0.194
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x1w
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.807	48.807
High resolution limit [Å]	2.645	2.645
Number of reflections	37731	37731
<I/σ(I)>	1.37	10.79
Completeness [%]	99.7	99.88
Redundancy	6
CC(1/2)	0.763	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M citric acid (pH 4) and 30 % PEG 6000