8CY3

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Compound 15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	81.242, 161.235, 229.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.880 - 2.650
R-factor	0.1932
Rwork	0.193
R-free	0.22490
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7lnj
RMSD bond length	0.003
RMSD bond angle	0.581
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	2.740
High resolution limit [Å]	2.650	2.650
Rpim		0.650
Number of reflections	86741	7430
<I/σ(I)>	8.6
Completeness [%]	97.3
Redundancy	11.3
CC(1/2)	0.993	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	21-24% PEG 3350, 0.1 M Tris-HCl pH 7-7.5, 0.28M Potassium Citrate