8CY1

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Compound 19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	81.248, 161.568, 229.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.890 - 2.380
R-factor	0.1792
Rwork	0.178
R-free	0.22600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7lnj
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.890	2.480
High resolution limit [Å]	2.380	2.390
Number of reflections	117552	9384
<I/σ(I)>	8
Completeness [%]	96.2
Redundancy	8.6
CC(1/2)	0.982	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	21-24% PEG 3350, 0.1 M Tris-HCl pH 7-7.5, 0.28M Potassium Citrate