8CXU

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Compound 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	81.446, 161.237, 229.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.860 - 2.280
R-factor	0.1879
Rwork	0.187
R-free	0.22070
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7lnj
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.860	2.370
High resolution limit [Å]	2.280	2.280
Rmerge	0.233	1.700
Rpim	0.072	0.733
Number of reflections	129038	10190
<I/σ(I)>	10	0.9
Completeness [%]	93.5	74.8
Redundancy	8.9
CC(1/2)		0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	21~24% (w/v) polyethylene glycol 3350, 0.1 M Tris-HCl pH 7.0~7.5, 0.28 M potassium citrate