8CUC
Crystal structure analysis of SALL4 zinc finger domain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.250, 70.510, 112.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.680 - 2.090 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23070 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.510 | 70.550 | 2.130 |
| High resolution limit [Å] | 2.090 | 5.670 | 2.090 |
| Rmeas | 0.232 | 0.097 | 2.343 |
| Rpim | 0.099 | 0.042 | 0.984 |
| Total number of observations | 124824 | 6340 | 6402 |
| Number of reflections | 23766 | 1319 | 1171 |
| <I/σ(I)> | 6.7 | 15.1 | 1.3 |
| Completeness [%] | 99.8 | 100 | 99.9 |
| Redundancy | 5.3 | 4.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG3350, 40 mM ammonium sulfate, Bis-Tris, pH 6.0 |
