8CUC

Crystal structure analysis of SALL4 zinc finger domain in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2827
Spacegroup name	P 21 21 21
Unit cell lengths	49.250, 70.510, 112.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.680 - 2.090
R-factor	0.2035
Rwork	0.202
R-free	0.23070
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	SOLVE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.510	70.550	2.130
High resolution limit [Å]	2.090	5.670	2.090
Rmeas	0.232	0.097	2.343
Rpim	0.099	0.042	0.984
Total number of observations	124824	6340	6402
Number of reflections	23766	1319	1171
<I/σ(I)>	6.7	15.1	1.3
Completeness [%]	99.8	100	99.9
Redundancy	5.3	4.8	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG3350, 40 mM ammonium sulfate, Bis-Tris, pH 6.0