8CTM
Crystal structure of the nucleoside hydrolase from Leishmania donovani.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.221, 79.111, 107.621 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 25.870 - 1.730 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ezr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.093 | 0.397 |
| Rpim | 0.057 | 0.264 |
| Number of reflections | 263968 | 6560 |
| <I/σ(I)> | 23.5 | 2.8 |
| Completeness [%] | 94.1 | 93.4 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.970 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 15% PEG 3350 0.1 M MES saturated hypoxanthine |
