8CSH

Structure of the DNA binding domain of pSK1 Par partition protein bound to centromere DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	70.377, 91.069, 59.184
Unit cell angles	90.00, 119.85, 90.00

Refinement procedure

Resolution	34.064 - 2.250
R-factor	0.207
Rwork	0.202
R-free	0.24920
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.360	2.330
High resolution limit [Å]	2.250	2.250
Rpim	0.056	0.045
Number of reflections	14410	1320
<I/σ(I)>	8	2.2
Completeness [%]	94.3
Redundancy	3
CC(1/2)	0.992	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	24% PEG 5000, 0.2 M Ammonium sulphate, 0.1 M Tris pH 7.5