8CRA
Structure of the keratin-like domain of SEPALLATA3 and AGAMOUS from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.335, 138.372, 180.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.2388 |
| Rwork | 0.237 |
| R-free | 0.27911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.331 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.097 | 1.630 |
| Number of reflections | 95018 | 6627 |
| <I/σ(I)> | 11.3 | 0.9 |
| Completeness [%] | 99.3 | 0.932 |
| Redundancy | 3.8 | |
| CC(1/2) | 0.999 | 0.347 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Tris, pH 8.0, 2M sodium formate |
