8CR4

Crystal structure of recombinant LasBArtif from Pseudomonas aeruginosa AZPAE14816

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.6888
Spacegroup name	P 1
Unit cell lengths	40.405, 40.464, 45.376
Unit cell angles	100.52, 98.61, 107.45

Refinement procedure

Resolution	21.770 - 0.910
R-factor	0.1951
Rwork	0.195
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.201
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.540	0.940
High resolution limit [Å]	0.910	0.910
Rmerge	0.072
Rmeas	0.088
Rpim	0.049
Number of reflections	340238	17196
<I/σ(I)>	9.3
Completeness [%]	94.6
Redundancy	3.2
CC(1/2)	0.986	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05 M potassium chloride, 0.01 M magnesium chloride, 15% (w/v) PEG 6000