8CQQ
Crystal structure of an 8-repeat consensus TPR superhelix with Copper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.37323 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.450, 55.450, 55.520 |
| Unit cell angles | 111.47, 111.13, 105.80 |
Refinement procedure
| Resolution | 45.770 - 2.600 |
| R-factor | 0.18198 |
| Rwork | 0.178 |
| R-free | 0.26124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.770 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.137 | 0.548 |
| Rmeas | 0.185 | 0.751 |
| Rpim | 0.107 | 0.432 |
| Total number of observations | 39734 | 5632 |
| Number of reflections | 14716 | 2115 |
| <I/σ(I)> | 5.3 | 2.5 |
| Completeness [%] | 94.1 | |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.959 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 10 mM CuSO4 100 mM NaOAc 30% MPD |
