8CQQ

Crystal structure of an 8-repeat consensus TPR superhelix with Copper

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.37323
Spacegroup name	P 1
Unit cell lengths	55.450, 55.450, 55.520
Unit cell angles	111.47, 111.13, 105.80

Refinement procedure

Resolution	45.770 - 2.600
R-factor	0.18198
Rwork	0.178
R-free	0.26124
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.423
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.770	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.137	0.548
Rmeas	0.185	0.751
Rpim	0.107	0.432
Total number of observations	39734	5632
Number of reflections	14716	2115
<I/σ(I)>	5.3	2.5
Completeness [%]	94.1
Redundancy	2.7	2.7
CC(1/2)	0.959	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	10 mM CuSO4 100 mM NaOAc 30% MPD