8CPL

YZw2 a scaffold for cryo-EM of small proteins of interest

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976254
Spacegroup name	P 21 21 21
Unit cell lengths	73.453, 173.903, 209.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.440 - 1.605
R-factor	0.1888
Rwork	0.188
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.440	1.751
High resolution limit [Å]	1.600	1.605
Rmerge	0.171	1.719
Rmeas	0.178	1.788
Rpim	0.048	0.485
Number of reflections	203302	10164
<I/σ(I)>	10	1.8
Completeness [%]	58.4	12.8
Redundancy	13.8	12.7
CC(1/2)	0.996	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16.6% w/v PEG3350, 0.2M NaF and 0.1M Bis-Tris Propane pH 5.5