8CPK
Crystal structure of EtpA secretion domain from Enterotoxigenic Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.711, 64.188, 123.957 |
| Unit cell angles | 91.61, 90.89, 90.02 |
Refinement procedure
| Resolution | 61.950 - 1.760 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.799 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.162 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 112100 | 5450 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.297 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 0.1 M Na2 citrate pH 5.5, 20% (w/v) PEG 4000, 20% (v/v) isopropanol |
