8CP8
Crystal structure of an 8-repeat consensus TPR superhelix with Lead
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.951147 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.257, 73.257, 117.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.900 - 1.680 |
| R-factor | 0.20555 |
| Rwork | 0.204 |
| R-free | 0.23401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.111 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.609 | 1.713 |
| High resolution limit [Å] | 1.680 | 1.684 |
| Rmerge | 0.276 | 3.890 |
| Rmeas | 0.284 | 4.700 |
| Rpim | 0.065 | 0.952 |
| Number of reflections | 42074 | 2098 |
| <I/σ(I)> | 6.9 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.8 | 17.1 |
| CC(1/2) | 0.993 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 100mM NaOAc 20mM PbCl2 30% MPD |
