8CP5
Structure of Aspartate-N-hydroxylase (FzmM)from Streptomyces sp. V2: complex with NADPH and Sulphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-09 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967700 |
| Spacegroup name | H 3 |
| Unit cell lengths | 243.681, 243.681, 126.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.980 - 2.540 |
| R-factor | 0.20603 |
| Rwork | 0.204 |
| R-free | 0.24181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.708 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.980 | 2.580 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.194 | 1.800 |
| Rmeas | 0.203 | 4.151 |
| Rpim | 0.061 | 1.266 |
| Total number of observations | 993652 | 40976 |
| Number of reflections | 91662 | 3985 |
| <I/σ(I)> | 10.1 | 0.6 |
| Completeness [%] | 99.1 | |
| Redundancy | 10.8 | 10.3 |
| CC(1/2) | 0.996 | 0.293 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Hepes pH 7 1.6 M Ammonium sulphate |
