8CP4
[4Fe-4S] cluster containing LarE in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 186.170, 212.690, 196.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.870 - 3.190 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS (Dec, 2020) |
| Data scaling software | XDS (Dec, 2020) |
| Phasing software | PHENIX (Jan 31, 2020) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.870 | 47.870 | 3.390 |
| High resolution limit [Å] | 3.190 | 9.420 | 3.190 |
| Rmeas | 0.163 | 0.077 | 2.100 |
| Number of reflections | 64718 | 2669 | 10166 |
| <I/σ(I)> | 7.6 | 25.26 | 0.77 |
| Completeness [%] | 99.6 | 98 | 98.2 |
| Redundancy | 7.8 | ||
| CC(1/2) | 0.996 | 0.995 | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 4.1 M NaCl, 0.1 M HEPES pH 7.5, 10 % Glycerol |
