8CP4

[4Fe-4S] cluster containing LarE in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	C 2 2 21
Unit cell lengths	186.170, 212.690, 196.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.870 - 3.190
R-factor	0.2182
Rwork	0.216
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.463
Data reduction software	XDS (Dec, 2020)
Data scaling software	XDS (Dec, 2020)
Phasing software	PHENIX (Jan 31, 2020)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.870	47.870	3.390
High resolution limit [Å]	3.190	9.420	3.190
Rmeas	0.163	0.077	2.100
Number of reflections	64718	2669	10166
<I/σ(I)>	7.6	25.26	0.77
Completeness [%]	99.6	98	98.2
Redundancy	7.8
CC(1/2)	0.996	0.995	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	4.1 M NaCl, 0.1 M HEPES pH 7.5, 10 % Glycerol