8CON
Crystal structure of alcohol dehydrogenase from Arabidopsis thaliana in complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.405, 63.405, 182.153 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.020 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.150 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.711 |
| Rmeas | 0.076 | 0.826 |
| Rpim | 0.035 | 0.411 |
| Number of reflections | 47829 | 2492 |
| <I/σ(I)> | 15.1 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 7.3 |
| CC(1/2) | 0.999 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Hepes-NaOH pH 7.0-8.0, 2% v/v PEG 400 and 2.0 M (NH4)2SO4 |
