8CO5
The surface-engineered photosensory module (PAS-GAF-PHY) of the bacterial phytochrome Agp1 (AtBphP1) in the Pr form with parallel dimer formation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918410 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 172.477, 172.477, 78.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.490 - 2.420 |
| R-factor | 0.21408 |
| Rwork | 0.212 |
| R-free | 0.25195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.490 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Number of reflections | 22938 | 2361 |
| <I/σ(I)> | 22 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.999 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 10-20% PEG4000, 100mM Tris buffer, 0.2M MgCl2 |
