8CNX
Structure of Enterovirus D68 3C protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-20 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9212 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.820, 62.530, 147.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.627 - 1.490 |
| Rwork | 0.204 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.581 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.630 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.099 | |
| Number of reflections | 60463 | 11079 |
| <I/σ(I)> | 11.4 | 0.7 |
| Completeness [%] | 91.9 | 56.7 |
| Redundancy | 10.5 | 4.1 |
| CC(1/2) | 0.998 | 0.388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.14 | 293 | 25% PEG 3,350 0.1 M Tris 0.2 M Ammonium acetate |
