8CN6
CD59 in complex with CP-06 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-10 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.940, 85.880, 96.570 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 48.332 - 2.430 |
| Rwork | 0.235 |
| R-free | 0.29830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.777 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.332 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.102 | 0.230 |
| Rmeas | 0.122 | 0.272 |
| Rpim | 0.066 | 0.143 |
| Number of reflections | 18920 | 1967 |
| <I/σ(I)> | 7 | 0.7 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.990 | 0.290 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.01 M ZnCl2, 0.1 M HEPES pH 7.0, 20% w/v PEG 6000 |
