8CN1
hDLG1-PDZ1 in complex with a TAX1 peptide from HTLV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.537, 82.905, 87.784 |
| Unit cell angles | 64.10, 90.14, 89.87 |
Refinement procedure
| Resolution | 45.250 - 2.090 |
| R-factor | 0.2016 |
| Rwork | 0.199 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.960 | 2.347 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.564 | |
| Rmeas | 0.665 | |
| Rpim | 0.350 | |
| Number of reflections | 49749 | 2488 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 89.3 | 10.4 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.990 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Bis-Tris pH 5.5, 0.18 M (NH4)2SO4, 24% PEG3350 |
