8CMP
DNA-binding bacterial histone protein HBB from Bdellovibrio bacteriovorus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 34.474, 56.345, 26.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.190 - 1.060 |
| R-factor | 0.14374 |
| Rwork | 0.142 |
| R-free | 0.16918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.034 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 1.130 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmeas | 0.047 | 0.921 |
| Number of reflections | 23226 | 3028 |
| <I/σ(I)> | 23.8 | 1.66 |
| Completeness [%] | 96.3 | 79.4 |
| Redundancy | 10.8 | 3.88 |
| CC(1/2) | 1.000 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium acetate, pH 4.5 and 25% PEG3350 |
